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biotinylated lineage markers  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec biotinylated lineage markers
    Biotinylated Lineage Markers, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lineage+markers/CD11b%2Fc+Antibody%2C+anti-rat%2C+REAfinity/pmc13113197-63-10-27
    Average 98 stars, based on 26 article reviews
    biotinylated lineage markers - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Selection:

    Article Title: α1,3-fucosylation treatment improves cord blood CD34 negative hematopoietic stem cell navigation
    Article Snippet: .. As a means of negative selection, we used a lineage cell depletion cocktail (Miltenyi Biotec) including ten lineage markers (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)) for lineage depletion. .. CB buffy coat-enriched cells were thawed in MEM-ɑ medium containing 10% ACD-A (anticoagulant citrate dextrose solution) and 5% human serum albumin (Sigma), centrifugated at 300g, resuspended in 10 mL PBS containing 0.1 mg/mL DNAse, incubated at room temperature for 10 minutes, centrifuged, washed twice with MEM medium containing 10% ACD-A, resuspended in 30 mL of MEM-ɑ culture medium with 5% FBS and incubated at 37°C for 12 to 24 hours in a hypoxic chamber (Stem cells Technologies) containing water in a petri dish.

    other:

    Article Title: SETDB1 suppresses NK cell-mediated immunosurveillance in acute myeloid leukemia with granulo-monocytic differentiation.
    Article Snippet: NK cells (CD49b+CD3e ) were sorted by BD FACSAriaIII (BD Biosciences) and cultured in RPMI-1640 containing 10% FBS (Biosera), 2-mercaptoethanol (50 mM, Gibco), HEPES (10 mM, Gibco), nonessential amino acid (Gibco), sodium pyruvate (Gibco), 10 ng/mL recombinant mouse IL-15 (PeproTech) and 100 U/ml mouse IL-2 (R&D Systems) for 5-day activation.69 For cytotoxicity assay, cSAM cells were transduced with sgRNA (coexpress puromycin) plasmids and performed the following procedures after 1 mg/mL puromycin selection for 4 days.

    Lysis:

    Article Title: Cohesin constrains histone modification-driven chromatin dynamics
    Article Snippet: .. Cell suspensions were lysed with erythrocyte lysis buffer (150 mM NH 4 Cl, 10 mM KHCO 3 , 100 μM EDTA-Na 2 ), filtered through a 40 μm filter, and incubated with a cocktail of biotinylated monoclonal antibodies to lineage markers (CD5, B220, CD11b, Gr-1, and Ter119) and then anti-biotin microbeads (Miltenyi Biotec). .. Lin − cells were purified using MACS separation LS columns (Miltenyi Biotec).

    Incubation:

    Article Title: Cohesin constrains histone modification-driven chromatin dynamics
    Article Snippet: .. Cell suspensions were lysed with erythrocyte lysis buffer (150 mM NH 4 Cl, 10 mM KHCO 3 , 100 μM EDTA-Na 2 ), filtered through a 40 μm filter, and incubated with a cocktail of biotinylated monoclonal antibodies to lineage markers (CD5, B220, CD11b, Gr-1, and Ter119) and then anti-biotin microbeads (Miltenyi Biotec). .. Lin − cells were purified using MACS separation LS columns (Miltenyi Biotec).

    Bioprocessing:

    Article Title: Cohesin constrains histone modification-driven chromatin dynamics
    Article Snippet: .. Cell suspensions were lysed with erythrocyte lysis buffer (150 mM NH 4 Cl, 10 mM KHCO 3 , 100 μM EDTA-Na 2 ), filtered through a 40 μm filter, and incubated with a cocktail of biotinylated monoclonal antibodies to lineage markers (CD5, B220, CD11b, Gr-1, and Ter119) and then anti-biotin microbeads (Miltenyi Biotec). .. Lin − cells were purified using MACS separation LS columns (Miltenyi Biotec).

    Staining:

    Article Title: Mycobacterium tuberculosis infection drives differential responses in the bone marrow hematopoietic stem and progenitor cells.
    Article Snippet: .. BM cells were harvested and stained as previously described, using the following antibodies from the Lineage Depletion Kit according to the manufacturer’s instructions (Miltenyi Biotec): lineage markers (CD3, CD5, Thy1.1, TER119, CD11b, Gr-1, and CD45R), LIVE/DEAD viability dye as above, cKit, and Sca1. .. To isolate the LKS+ population, the Lin−cKit+Sca1+ cells were sorted using the BD FACSJazz flow cytometer with FACS Sortware software, as outlined in Fig. 4A (BD).

    Article Title: Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions
    Article Snippet: .. A portion of BM cells was stained with biotinylated antibodies to lineage markers (Ter119, Gr-1, NK1.1, CD11b, B220 and TCRb) and negatively selected using streptavidin magnetic beads and LD MACS cell separation columns (Miltenyi Biotech). ..

    Magnetic Beads:

    Article Title: A Bcl11b N797K variant isolated from an immunodeficient patient inhibits early thymocyte development in mice
    Article Snippet: Frozen fetal liver (FL) samples were thawed and cultured in OP9 media (alpha MEM [Gibco] supplemented with 20% FBS, 50μM β-ME (Sigma), penicillin-streptomycin-glutamine supplement [Gibco]) overnight with 10 ng/ml IL-7 and Flt3L and 5 ng/ml KitL (Peprotech). .. The following day, cells were depleted by biotinylated antibodies (Biolegend) and anti-biotin magnetic beads (Miltenyi) for lineage markers (TCRβ, CD19, CD11c, NK1.1, Ter119, γδTCR, Gr-1). ..

    Article Title: Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions
    Article Snippet: .. A portion of BM cells was stained with biotinylated antibodies to lineage markers (Ter119, Gr-1, NK1.1, CD11b, B220 and TCRb) and negatively selected using streptavidin magnetic beads and LD MACS cell separation columns (Miltenyi Biotech). ..

    Magnetic Cell Separation:

    Article Title: Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions
    Article Snippet: .. A portion of BM cells was stained with biotinylated antibodies to lineage markers (Ter119, Gr-1, NK1.1, CD11b, B220 and TCRb) and negatively selected using streptavidin magnetic beads and LD MACS cell separation columns (Miltenyi Biotech). ..



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